Depletion of Kcnq1ot1 non-coding RNA does not affect imprinting maintenance in stem cells.

ABSTRACT: To understand the complex regulation of genomic imprinting it is important to determine how early embryos establish imprinted gene expression across large chromosomal domains. Long non-coding RNAs (ncRNAs) have been associated with the regulation of imprinting domains, yet their function remains undefined. Here, we investigated the mouse Kcnq1ot1 ncRNA and its role in imprinted gene regulation during preimplantation development by utilizing mouse embryonic and extra-embryonic stem cell models. Our findings demonstrate that the Kcnq1ot1 ncRNA extends 471 kb from the transcription start site. This is significant as it raises the possibility that transcription through downstream genes might play a role in their silencing, including Th, which we demonstrate possesses maternal-specific expressi

SUBMITTER: Golding MC 

PROVIDER: S-EPMC3152924 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature