Label-free protein profiling of adipose-derived human stem cells under hyperosmotic treatment.

ABSTRACT: Our previous work suggested that treatment of cells with hyperosmotic media during 2D passaging primes cells for cartilage tissue engineering applications. Here, we used label-free proteomic profiling to evaluate the effects of control and hyperosmotic treatment environments on the phenotype of multipotent adipose-derived stem cells (ASCs) cultivated with a chondrogenic growth factor cocktail. Spectra were recorded in a data-independent fashion at alternate low (precursor) and high (product) fragmentation voltages (MS(E)). This method was supplemented with data mining of accurate mass and retention time matches in precursor ion spectra across the experiment. The results indicated a complex cellular response to osmotic treatment, with a number of proteins differentially expressed between co

SUBMITTER: Oswald ES 

PROVIDER: S-EPMC3153440 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature