Aquaporin-facilitated water uptake in barley (Hordeum vulgare L.) roots.

ABSTRACT: It is not known to what degree aquaporin-facilitated water uptake differs between root developmental regions and types of root. The aim of this study was to measure aquaporin-dependent water flow in the main types of root and root developmental regions of 14- to 17-d-old barley plants and to identify candidate aquaporins which mediate this flow. Water flow at root level was related to flow at cell and plant level. Plants were grown hydroponically. Hydraulic conductivity of cells and roots was determined with a pressure probe and through exudation, respectively, and whole-plant water flow (transpiration) determined gravimetrically in response to the commonly used aquaporin inhibitor HgCl(2). Expression of aquaporins was analysed by real-time PCR and in situ hybridization. Hydraulic conducti

SUBMITTER: Knipfer T 

PROVIDER: S-EPMC3153672 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature