Developmental pattern of aquaporin expression in barley (Hordeum vulgare L.) leaves.

ABSTRACT: Aquaporins are multifunctional membrane channels which belong to the family of major intrinsic proteins (MIPs) and are best known for their ability to facilitate the movement of water. In the present study, earlier results from microarray experiments were followed up. These experiments had suggested that, in barley (Hordeum vulgare L.), aquaporin family members are expressed in distinct patterns during leaf development. Real-time PCR and in situ hybridization were used to analyse the level and tissue-distribution of expression of candidate aquaporins, focusing on plasma membrane and tonoplast intrinsic proteins (PIPs, TIPs). Water channel function of seven aquaporins, whose transcripts were the most abundant and the most variable, was tested through expression in yeast and, in part, throug

SUBMITTER: Besse M 

PROVIDER: S-EPMC3153690 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature