Dynamic membrane remodeling at invadopodia differentiates invadopodia from podosomes.

ABSTRACT: Invadopodia are specialized actin-rich protrusions of metastatic tumor and transformed cells with crucial functions in ECM degradation and invasion. Although early electron microscopy studies described invadopodia as long filament-like protrusions of the cell membrane adherent to the matrix, fluorescence microscopy studies have focused on invadopodia as actin-cortactin aggregates localized to areas of ECM degradation. The absence of a clear conceptual integration of these two descriptions of invadopodial structure has impeded understanding of the regulatory mechanisms that govern invadopodia. To determine the relationship between the membrane filaments identified by electron microscopy and the actin-cortactin aggregates of invadopodia, we applied rapid live-cell high-resolution TIRF micros

SUBMITTER: Artym VV 

PROVIDER: S-EPMC3153956 | biostudies-literature | 2011 Feb-Mar

REPOSITORIES: biostudies-literature