Diverse environmental stresses elicit distinct responses at the level of pre-mRNA processing in yeast.

ABSTRACT: Gene expression in eukaryotic cells is profoundly influenced by the post-transcriptional processing of mRNAs, including the splicing of introns in the nucleus and both nuclear and cytoplasmic degradation pathways. These processes have the potential to affect both the steady-state levels and the kinetics of changes to levels of intron-containing transcripts. Here we report the use of a splicing isoform-specific microarray platform to investigate the effects of diverse stress conditions on pre-mRNA processing. Interestingly, we find that diverse stresses cause distinct patterns of changes at this level. The responses we observed are most dramatic for the RPGs and can be categorized into three major classes. The first is characterized by accumulation of RPG pre-mRNA and is seen in multiple ty

SUBMITTER: Bergkessel M 

PROVIDER: S-EPMC3153971 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature