Live imaging of bulk endocytosis in frog motor nerve terminals using FM dyes.

ABSTRACT: We observed endocytosis in real time in stimulated frog motor nerve terminals by imaging the growth of large membrane infoldings labeled with a low concentration of FM dye. The spatial and temporal information made available by these experiments allowed us to image several new aspects of this synaptic vesicle recycling pathway. Membrane infoldings appeared near synaptic vesicle clusters and grew rapidly during long-duration, high-frequency stimulation. In some cases, we observed large, elongated infoldings growing laterally into the terminal. We used these observations to calculate infolding growth rates. A decrease in stimulation frequency caused a decrease in growth rates, but the overall length of these structures was unaffected by frequency changes. Attempts to wash the dye from these

SUBMITTER: Gaffield MA 

PROVIDER: S-EPMC3154819 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature