Complement component C5a promotes expression of IL-22 and IL-17 from human T cells and its implication in age-related macular degeneration.

ABSTRACT:

Background

Age related macular degeneration (AMD) is the leading cause of irreversible blindness in elderly populations worldwide. Inflammation, among many factors, has been suggested to play an important role in AMD pathogenesis. Recent studies have demonstrated a strong genetic association between AMD and complement factor H (CFH), the down-regulatory factor of complement activation. Elevated levels of complement activating molecules including complement component 5a (C5a) have been found in the serum of AMD patients. Our aim is to study whether C5a can impact human T cells and its implication in AMD.

Methods

Human peripheral blood mononuclear cells (PBMCs) were isolated from the blood of exudative form of AMD patients using a Ficoll gradient centrifugation protocol. Intra

SUBMITTER: Liu B 

PROVIDER: S-EPMC3154861 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature