Visual detection of H3 subtype avian influenza viruses by reverse transcription loop-mediated isothermal amplification assay.

ABSTRACT:

Background

Recent epidemiological investigation of different HA subtypes of avian influenza viruses (AIVs) shows that the H3 subtype is the most predominant among low pathogenic AIVs (LPAIVs), and the seasonal variations in isolation of H3 subtype AIVs are consistent with that of human H3 subtype influenza viruses. Consequently, the development of a rapid, simple, sensitive detection method for H3 subtype AIVs is required. The loop-mediated isothermal amplification (LAMP) assay is a simple, rapid, sensitive and cost-effective nucleic acid amplification method that does not require any specialized equipment.

Results

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to detect the H3 subtype AIVs visually. Specific primer sets target t

SUBMITTER: Peng Y 

PROVIDER: S-EPMC3154870 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature