Characterization of the small RNA transcriptome of the diatom, Thalassiosira pseudonana.

ABSTRACT: This study presents the first characterization of endogenous small RNAs in a diatom, Thalassiosira pseudonana. Small RNAs act as transcriptional and translational regulators, controlling specific target genes involved in various cellular functions. Diatoms are unicellular photosynthetic organisms that play major roles in environmental processes, such as food webs and global carbon fixation. Small RNA cDNA libraries were constructed for exponentially growing T. pseudonana, and then subjected to highly parallel pyrosequencing (454) and sequencing-by-ligation (Applied Biosystems SOLiD). From the computational analysis of approximately 300,000 sequences in the 454 library and over 17 million sequences in the SOLiD libraries, there exists evidence of a core set of small RNA genes including: nov

SUBMITTER: Norden-Krichmar TM 

PROVIDER: S-EPMC3155517 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature