Cellular expression of Smarca4 (Brg1)-regulated genes in zebrafish retinas.

ABSTRACT:

Background

In a recent genomic study, Leung et al. used a factorial microarray analysis to identify Smarca4 (Brg1)-regulated genes in micro-dissected zebrafish retinas. Two hundred and fifty nine genes were grouped in three-way ANOVA models which carried the most specific retinal change. To validate the microarray results and to elucidate cellular expression patterns of the significant genes for further characterization, 32 known genes were randomly selected from this group. In situ hybridization of these genes was performed on the same types of samples (wild-type (WT) and smarca4a50/a50 (yng) mutant) at the same stages (36 and 52 hours post-fertilization (hpf)) as in the microarray study.

Results

Thirty out of 32 riboprobes showed a positive in situ staining signal. Twenty

SUBMITTER: Hensley MR 

PROVIDER: S-EPMC3155967 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature