Inhibitor of kappaB kinase beta regulates gastric carcinogenesis via interleukin-1alpha expression.

ABSTRACT:

Background & aims

Nuclear factor-kappaB (NF-kappaB) is an important transcription factor involved in various biological processes, including carcinogenesis. However, it is unknown whether NF-kappaB activation is involved in gastric carcinogenesis.

Methods

To explore the roles of inhibitor of kappaB kinase (IKKbeta), the key kinase for NF-kappaB activation, in gastric epithelium, we established a conditional gastric mucosal epithelium knockout mouse (Ikkbeta(DeltaST)). Gastric cancer was induced using N-methyl-N-nitrosourea (MNU). After 8 months, the number of tumors and their sizes were evaluated. Apoptosis was analyzed by terminal deoxynucleotidyl transferase-mediated deoxyuridine nick-end labeling staining, and levels of inflammatory cytokines were measured.

Results

SUBMITTER: Sakamoto K 

PROVIDER: S-EPMC3156098 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature