Rapid probing of biological surfaces with a sparse-matrix peptide library.

ABSTRACT: Finding unique peptides to target specific biological surfaces is crucial to basic research and technology development, though methods based on biological arrays or large libraries limit the speed and ease with which these necessary compounds can be found. We reasoned that because biological surfaces, such as cell surfaces, mineralized tissues, and various extracellular matrices have unique molecular compositions, they present unique physicochemical signatures to the surrounding medium which could be probed by peptides with appropriately corresponding physicochemical properties. To test this hypothesis, a naïve pilot library of 36 peptides, varying in their hydrophobicity and charge, was arranged in a two-dimensional matrix and screened against various biological surfaces. While the number

SUBMITTER: Yarbrough DK 

PROVIDER: S-EPMC3156232 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature