A solid phase extraction-based platform for rapid phosphoproteomic analysis.

ABSTRACT: Protein phosphorylation is among the most common and intensely studied post-translational protein modification. It plays crucial roles in virtually all cellular processes and has been implicated in numerous human diseases, including cancer. Traditional biochemical and genetic methods for identifying and monitoring sites of phosphorylation are laborious and slow and in recent years have largely been replaced by mass spectrometric analysis. Improved methods for phosphopeptide enrichment coupled with faster and more sensitive mass spectrometers have led to an explosion in the size of phosphoproteomic datasets. However, wider application of these methods is limited by equipment costs and the resultant high demand for instrument time as well as by a technology gap between biologists and mass sp

SUBMITTER: Dephoure N 

PROVIDER: S-EPMC3156280 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature