Non-invasive, dynamic imaging of murine intestinal motility.

ABSTRACT:

Background

After intravenous (i.v.) administration, indocyanine green (ICG) is known to be secreted into bile from the liver via the biliary tracts, enabling fluorescent delineation of the intestine. In addition, ICG is a near-infrared (NIR) excitable fluorophore, capable of providing exogenous contrast for rapid NIR fluorescence imaging. We sought to quantify the intestinal motility using dynamic NIR fluorescence imaging after injection of ICG.

Methods

C57BL6 mice were dynamically imaged immediately before and up to 24h after i.v. and intradermal (i.d.) injection of 50 and 10μL of ICG, respectively. Necropsy was also performed 1h postinjection and the entire gastrointestinal tract was isolated and exposed for ex vivo fluorescence imaging.

Key results

The secretion of

SUBMITTER: Kwon S 

PROVIDER: S-EPMC3156289 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature