Evaluation of FKBP and DHFR based destabilizing domains in Saccharomyces cerevisiae.

ABSTRACT: Two orthogonal destabilizing domains have been developed based on mutants of human FKBP12 as well as bacterial DHFR and these engineered domains have been used to control protein concentration in a variety of contexts in vitro and in vivo. FKBP12 based destabilizing domains cannot be rescued in the yeast Saccharomyces cerevisiae; ecDHFR based destabilizing domains are not degraded as efficiently in S. cerevisiae as in mammalian cells or Plasmodium, but provide a starting point for the development of domains with increased signal-to-noise in S. cerevisiae.

SUBMITTER: Rakhit R 

PROVIDER: S-EPMC3156383 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature