Properties of procoagulant platelets: defining and characterizing the subpopulation binding a functional prothrombinase.

ABSTRACT:

Objective

The goal of this study was to define and characterize the subpopulation of platelets capable of regulating the functional interactions of factors Va (FVa) and Xa (FXa) on the thrombin-activated platelet surface.

Methods and results

Flow cytometric analyses were used to define and characterize platelet subpopulations. At a concentration of thrombin known to elicit maximal platelet activation, platelet-derived FVa release, and prothrombinase assembly/function, only a subpopulation of platelets was positive for FVa and FXa binding. An additional subpopulation bound lower levels of FVa but little, if any, FXa. Fluorescence microscopy analyses confirmed these data. Phenotypically, platelets capable of binding FXa were more highly reticulated and demonstrated significant

SUBMITTER: Fager AM 

PROVIDER: S-EPMC3156579 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature