Sequential association of myogenic regulatory factors and E proteins at muscle-specific genes.

ABSTRACT:

Background

Gene expression in skeletal muscle is controlled by a family of basic helix-loop-helix transcription factors known as the myogenic regulatory factors (MRFs). The MRFs work in conjunction with E proteins to regulate gene expression during myogenesis. However, the precise mechanism by which the MRFs activate gene expression is unclear. In this work, we sought to define the binding profiles of MRFs and E proteins on muscle-specific genes throughout a time course of differentiation.

Results

We performed chromatin immunoprecipitation (ChIP) assays for myogenin, MyoD, Myf5 and E proteins over a time course of C2C12 differentiation, resulting in several surprising findings. The pattern of recruitment is specific to each promoter tested. The recruitment of E proteins ofte

SUBMITTER: Londhe P 

PROVIDER: S-EPMC3156637 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature