Integrated genomics and proteomics of the Torpedo californica electric organ: concordance with the mammalian neuromuscular junction.

ABSTRACT:

Background

During development, the branchial mesoderm of Torpedo californica transdifferentiates into an electric organ capable of generating high voltage discharges to stun fish. The organ contains a high density of cholinergic synapses and has served as a biochemical model for the membrane specialization of myofibers, the neuromuscular junction (NMJ). We studied the genome and proteome of the electric organ to gain insight into its composition, to determine if there is concordance with skeletal muscle and the NMJ, and to identify novel synaptic proteins.

Results

Of 435 proteins identified, 300 mapped to Torpedo cDNA sequences with ≥2 peptides. We identified 14 uncharacterized proteins in the electric organ that are known to play a role in acetylcholine receptor clustering

SUBMITTER: Mate SE 

PROVIDER: S-EPMC3156643 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature