Cyclen-based cationic lipids for highly efficient gene delivery towards tumor cells.

ABSTRACT:

Background

Gene therapy has tremendous potential for both inherited and acquired diseases. However, delivery problems limited their clinical application, and new gene delivery vehicles with low cytotoxicity and high transfection efficiency are greatly required.

Methods

In this report, we designed and synthesized three amphiphilic molecules (L1-L3) with the structures involving 1, 4, 7, 10-tetraazacyclododecane (cyclen), imidazolium and a hydrophobic dodecyl chain. Their interactions with plasmid DNA were studied via electrophoretic gel retardation assays, fluorescent quenching experiments, dynamic light scattering and transmission electron microscopy. The in vitro gene transfection assay and cytotoxicity assay were conducted in four cell lines.

Results

Results indicat

SUBMITTER: Huang QD 

PROVIDER: S-EPMC3156682 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature