Parentage determination of Vanda Miss Joaquim (Orchidaceae) through two chloroplast genes rbcL and matK.

ABSTRACT:

Background and aims

The popular hybrid orchid Vanda Miss Joaquim was made Singapore's national flower in 1981. It was originally described in the Gardeners' Chronicle in 1893, as a cross between Vanda hookeriana and Vanda teres. However, no record had been kept as to which parent contributed the pollen. This study was conducted using DNA barcoding techniques to determine the pod parent of V. Miss Joaquim, thereby inferring the pollen parent of the hybrid by exclusion.

Methodology

Two chloroplast genes, matK and rbcL, from five related taxa, V. hookeriana, V. teres var. alba, V. teres var. andersonii, V. teres var. aurorea and V. Miss Joaquim 'Agnes', were sequenced. The matK gene from herbarium specimens of V. teres and V. Miss Joaquim, both collected in 1893, was also seque

SUBMITTER: Khew GS 

PROVIDER: S-EPMC3156982 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature