Evidence for coregulation of myocardial gene expression by MEF2 and NFAT in human heart failure.

ABSTRACT:

Background

Pathological stresses induce heart failure in animal models through activation of multiple cardiac transcription factors (TFs) working cooperatively. However, interactions among TFs in human heart failure are less understood. Here, we use genomic data to examine the evidence that 5 candidate TF families coregulate gene expression in human heart failure.

Methods and results

RNA isolates from failing (n=86) and nonfailing (n=16) human hearts were hybridized with Affymetrix HU133A arrays. For each gene on the array, we determined conserved MEF2, NFAT, NKX , GATA , and FOX binding motifs within the -1-kb promoter region using human-murine sequence alignments and the TRANSFAC database. Across 9076 genes expressed in the heart, TF-binding motifs tended to cluster togeth

SUBMITTER: Putt ME 

PROVIDER: S-EPMC3157251 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature