MIRTFnet: analysis of miRNA regulated transcription factors.

ABSTRACT:

Background

Several expression datasets of miRNA transfection experiments are available to analyze the regulatory mechanisms downstream of miRNA effects. The miRNA induced regulatory effects can be propagated via transcription factors (TFs). We propose the method MIRTFnet to identify miRNA controlled TFs as active regulators if their downstream target genes are differentially expressed.

Methodology/principal findings

MIRTFnet enables the determination of active transcription factors (TFs) and is sensitive enough to exploit the small expression changes induced by the activity of miRNAs. For this purpose, different statistical tests were evaluated and compared. Based on the identified TFs, databases, computational predictions and the literature we construct regulatory models do

SUBMITTER: Naeem H 

PROVIDER: S-EPMC3157336 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature