Genomic analysis of parent-of-origin allelic expression in Arabidopsis thaliana seeds.

ABSTRACT: Differential expression of maternally and paternally inherited alleles of a gene is referred to as gene imprinting, a form of epigenetic gene regulation common to flowering plants and mammals. In plants, imprinting primarily occurs in the endosperm, a seed tissue that supports the embryo during its growth and development. Previously, we demonstrated that widespread DNA demethylation at remnants of transposable elements accompanies endosperm development and that a subset of these methylation changes are associated with gene imprinting. Here we assay imprinted gene expression genome-wide by performing high-throughput sequencing of RNA derived from seeds of reciprocal intraspecific crosses. We identify more than 200 loci that exhibit parent-of-origin effects on gene expression in the endosper

SUBMITTER: Gehring M 

PROVIDER: S-EPMC3157454 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature