Photochemical control of DNA decoy function enables precise regulation of nuclear factor κB activity.

ABSTRACT: DNA decoys have been developed for the inhibition of transcriptional regulation of gene expression. However, the present methodology lacks the spatial and temporal control of gene expression that is commonly found in nature. Here, we report the application of photoremovable protecting groups on nucleobases of nuclear factor κB (NF-κB) DNA decoys to regulate NF-κB-driven transcription of secreted alkaline phosphatase using light as an external control element. The NF-κB family of proteins is comprised of important eukaryotic transcription factors that regulate a wide range of cellular processes and are involved in immune response, development, cellular growth, and cell death. Several diseases, including cancer, arthritis, chronic inflammation, asthma, neurodegenerative diseases, and heart d

SUBMITTER: Govan JM 

PROVIDER: S-EPMC3157586 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature