An EMT-driven alternative splicing program occurs in human breast cancer and modulates cellular phenotype.

ABSTRACT: Epithelial-mesenchymal transition (EMT), a mechanism important for embryonic development, plays a critical role during malignant transformation. While much is known about transcriptional regulation of EMT, alternative splicing of several genes has also been correlated with EMT progression, but the extent of splicing changes and their contributions to the morphological conversion accompanying EMT have not been investigated comprehensively. Using an established cell culture model and RNA-Seq analyses, we determined an alternative splicing signature for EMT. Genes encoding key drivers of EMT-dependent changes in cell phenotype, such as actin cytoskeleton remodeling, regulation of cell-cell junction formation, and regulation of cell migration, were enriched among EMT-associated alternatively s

SUBMITTER: Shapiro IM 

PROVIDER: S-EPMC3158048 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature