Hyperthermia induces the ER stress pathway.

ABSTRACT:

Background

The ER chaperone GRP78/BiP is a homolog of the Hsp70 family of heat shock proteins, yet GRP78/BiP is not induced by heat shock but instead by ER stress. However, previous studies had not considered more physiologically relevant temperature elevation associated with febrile hyperthermia. In this report we examine the response of GRP78/BiP and other components of the ER stress pathway in cells exposed to 40°C.

Methodology

AD293 cells were exposed to 43°C heat shock to confirm inhibition of the ER stress response genes. Five mammalian cell types, including AD293 cells, were then exposed to 40°C hyperthermia for various time periods and induction of the ER stress pathway was assessed.

Principal findings

The inhibition of the ER stress pathway by heat shock (43°

SUBMITTER: Xu X 

PROVIDER: S-EPMC3158104 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature