Phenotype-information-phenotype cycle for deconvolution of combinatorial antibody libraries selected against complex systems.

ABSTRACT: Use of large combinatorial antibody libraries and next-generation sequencing of nucleic acids are two of the most powerful methods in modern molecular biology. The libraries are screened using the principles of evolutionary selection, albeit in real time, to enrich for members with a particular phenotype. This selective process necessarily results in the loss of information about less-fit molecules. On the other hand, sequencing of the library, by itself, gives information that is mostly unrelated to phenotype. If the two methods could be combined, the full potential of very large molecular libraries could be realized. Here we report the implementation of a phenotype-information-phenotype cycle that integrates information and gene recovery. After selection for phage-encoded antibodies that

SUBMITTER: Zhang H 

PROVIDER: S-EPMC3158238 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature