An EST-SSR linkage map of Raphanus sativus and comparative genomics of the Brassicaceae.

ABSTRACT: Raphanus sativus (2n = 2x = 18) is a widely cultivated member of the family Brassicaceae, for which genomic resources are available only to a limited extent in comparison to many other members of the family. To promote more genetic and genomic studies and to enhance breeding programmes of R. sativus, we have prepared genetic resources such as complementary DNA libraries, expressed sequences tags (ESTs), simple sequence repeat (SSR) markers and a genetic linkage map. A total of 26 606 ESTs have been collected from seedlings, roots, leaves, and flowers, and clustered into 10 381 unigenes. Similarities were observed between the expression patterns of transcripts from R. sativus and those from representative members of the genera Arabidopsis and Brassica, indicating their functional relatednes

SUBMITTER: Shirasawa K 

PROVIDER: S-EPMC3158468 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature