APC(cdh1) mediates degradation of the oncogenic Rho-GEF Ect2 after mitosis.

ABSTRACT:

Background

Besides regulation of actin cytoskeleton-dependent functions, Rho GTPase pathways are essential to cell cycle progression and cell division. Rho, Rac and Cdc42 regulate G1 to S phase progression and are involved in cytokinesis. RhoA GDP/GTP cycling is required for normal cytokinesis and recent reports have shown that the exchange factor Ect2 and the GTPase activating protein MgcRacGAP regulate RhoA activity during mitosis. We previously showed that the transcription factors E2F1 and CUX1 regulate expression of MgcRacGAP and Ect2 as cells enter S-phase.

Methodology/principal findings

We now report that Ect2 is subject to proteasomal degradation after mitosis, following ubiquitination by the APC/C complex and its co-activator Cdh1. A proper nuclear localization of E

SUBMITTER: Liot C 

PROVIDER: S-EPMC3158779 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature