Nucleoplasmic calcium regulates cell proliferation through legumain.

ABSTRACT:

Background & aims

Nucleoplasmic Ca(2+) regulates cell growth in the liver, but the proteins through which this occurs are unknown.

Methods

We used Rapid Subtraction Hybridization (RaSH) to subtract genes in SKHep1 liver cells expressing the Ca(2+) buffer protein parvalbumin (PV) targeted to the nucleus, from genes in cells expressing a mutated form of nuclear-targeted PV which has one of two Ca(2+)-binding sites inactivated. The subtraction permitted the selection of genes whose expression was affected by a small alteration in nuclear Ca(2+) concentration.

Results

The asparaginyl endopeptidase legumain (LGMN) was identified in this screening. When Ca(2+) was buffered in the nucleus of SKHep1 cells, LGMN mRNA was decreased by 97%, in part by a transcriptional mechanism

SUBMITTER: Andrade V 

PROVIDER: S-EPMC3158841 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature