The role of tRNA and ribosome competition in coupling the expression of different mRNAs in Saccharomyces cerevisiae.

ABSTRACT: Protein synthesis translates information from messenger RNAs into functional proteomes. Because of the finite nature of the resources required by the translational machinery, both the overall protein synthesis activity of a cell and activity on individual mRNAs are controlled by the allocation of limiting resources. Upon introduction of heterologous sequences into an organism-for example for the purposes of bioprocessing or synthetic biology-limiting resources may also become overstretched, thus negatively affecting both endogenous and heterologous gene expression. In this study, we present a mean-field model of translation in Saccharomyces cerevisiae for the investigation of two particular translational resources, namely ribosomes and aminoacylated tRNAs. We firstly use comparisons of exp

SUBMITTER: Chu D 

PROVIDER: S-EPMC3159466 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature