HBV vaccine efficacy and detection and genotyping of vaccinee asymptomatic breakthrough HBV infection in Egypt.

ABSTRACT:

Aim

To evaluate the impact of mass vaccination against the hepatitis B virus (HBV) in Egypt, and to search for vaccinee asymptomatic breakthrough HBV infection and its genotype.

Methods

Seven hundred serum samples from vaccinated children and adults (aged 2-47 years) were used for quantitative and qualitative detection of HBsAb by ELISA. Three hundred and sixty serum samples representing undetectable or low or high HBsAb were screened for markers of active HBV infection (HBsAg, HBcAb (IgG) and HBeAb by ELISA, plus HBsAg by AxSYM) and HBV-DNA genotyping by nested multiplex PCR and by DNA sequencing.

Results

It was found that 65% of children aged 2-4 years, and 20.5% aged 4-13 years, as well as 45% adults were good responders to HBV vaccination mounting protective level

SUBMITTER: Abushady EA 

PROVIDER: S-EPMC3159495 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature