Hypoxia-induced inflammatory cytokine secretion in human adipose tissue stromovascular cells.

ABSTRACT:

Aims

Hypoxia has been implicated as a cause of adipose tissue inflammation in obesity, although the inflammatory response of human adipose tissue to hypoxia is not well understood. The goal of this study was to define in vitro inflammatory responses of human adipose tissue to hypoxia and identify molecular mechanisms of hypoxia-induced inflammation.

Methods

The inflammatory milieu and responses of visceral (VAT) and subcutaneous (SAT) adipose tissue explants and purified stromovascular cells (SVFs) from obese and lean humans were studied in an in vitro hypoxic culture system using quantitative real-time PCR, ELISA, western blotting, immunofluorescence microscopy, flow cytometry and immunohistochemistry.

Results

Human adipose tissue in obesity demonstrates an increased

SUBMITTER: O'Rourke RW 

PROVIDER: S-EPMC3159546 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature