False positive circumsporozoite protein ELISA: a challenge for the estimation of the entomological inoculation rate of malaria and for vector incrimination.

ABSTRACT:

Background

The entomological inoculation rate (EIR) is an important indicator in estimating malaria transmission and the impact of vector control. To assess the EIR, the enzyme-linked immunosorbent assay (ELISA) to detect the circumsporozoite protein (CSP) is increasingly used. However, several studies have reported false positive results in this ELISA. The false positive results could lead to an overestimation of the EIR. The aim of present study was to estimate the level of false positivity among different anopheline species in Cambodia and Vietnam and to check for the presence of other parasites that might interact with the anti-CSP monoclonal antibodies.

Methods

Mosquitoes collected in Cambodia and Vietnam were identified and tested for the presence of sporozoites in hea

SUBMITTER: Durnez L 

PROVIDER: S-EPMC3160429 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature