Hepatitis B virus and hepatocellular carcinoma at the miRNA level.

ABSTRACT:

Aim

To study Hepatitis B virus (HBV) infection and its association with hepatocellular carcinoma (HCC) at the miRNA level.

Methods

Three cellular models were used to investigate miRNA expression changes during HBV infection: human HepG2 hepatoblastoma cell line as a model without HBV infection; HepG2 cell line transfected with a 1.3-fold full-length HBV genome as an acute infection model; and HepG2.2.15 cell line, which is derived from HepG2 and stably transfected with a complete HBV genome, as a chronic infection model. The miRNA levels were examined using microarray technology. To explore the relationship between HBV infection and HCC genesis at the miRNA level, we downloaded from national center for biotechnology information Gene Expression Omnibus an miRNA expression dat

SUBMITTER: Zhang ZZ 

PROVIDER: S-EPMC3160541 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature