Cytoskeletal control of CD36 diffusion promotes its receptor and signaling function.

ABSTRACT: The mechanisms that govern receptor coalescence into functional clusters--often a critical step in their stimulation by ligand--are poorly understood. We used single-molecule tracking to investigate the dynamics of CD36, a clustering-responsive receptor that mediates oxidized LDL uptake by macrophages. We found that CD36 motion in the membrane was spatially structured by the cortical cytoskeleton. A subpopulation of receptors diffused within linear confinement regions whose unique geometry simultaneously facilitated freedom of movement along one axis while increasing the effective receptor density. Co-confinement within troughs enhanced the probability of collisions between unligated receptors and promoted their clustering. Cytoskeleton perturbations that inhibited diffusion in linear conf

SUBMITTER: Jaqaman K 

PROVIDER: S-EPMC3160624 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature