Functional characterization of the sciarid BhC4-1 core promoter in transgenic Drosophila.

ABSTRACT:

Background

Core promoters are cis-regulatory modules to which bind the basal transcriptional machinery and which participate in the regulation of transcription initiation. Although core promoters have not been extensively investigated through functional assays in a chromosomal context, the available data suggested that the response of a given core promoter might vary depending on the promoter context. Previous studies suggest that a (-57/+40) fragment constitutes the core promoter of the BhC4-1 gene which is located in DNA puff C4 of the sciarid fly Bradysia hygida. Here we tested this (-57/+40) fragment in distinct regulatory contexts in order to verify if promoter context affects its core promoter activity.

Results

Consistent with the activity of a core promoter, we showed

SUBMITTER: Garcia AC 

PROVIDER: S-EPMC3160885 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature