Open-target sparse sensing of biological agents using DNA microarray.

ABSTRACT:

Background

Current biosensors are designed to target and react to specific nucleic acid sequences or structural epitopes. These 'target-specific' platforms require creation of new physical capture reagents when new organisms are targeted. An 'open-target' approach to DNA microarray biosensing is proposed and substantiated using laboratory generated data. The microarray consisted of 12,900 25 bp oligonucleotide capture probes derived from a statistical model trained on randomly selected genomic segments of pathogenic prokaryotic organisms. Open-target detection of organisms was accomplished using a reference library of hybridization patterns for three test organisms whose DNA sequences were not included in the design of the microarray probes.

Results

A multivariate mathematic

SUBMITTER: Mohtashemi M 

PROVIDER: S-EPMC3161048 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature