Islet amyloid polypeptide is a target antigen for diabetogenic CD4+ T cells.

ABSTRACT:

Objective

To investigate autoantigens in β-cells, we have used a panel of pathogenic T-cell clones that were derived from the NOD mouse. Our particular focus in this study was on the identification of the target antigen for the highly diabetogenic T-cell clone BDC-5.2.9.

Research design and methods

To purify β-cell antigens, we applied sequential size exclusion chromatography and reverse-phase high-performance liquid chromatography to membrane preparations of β-cell tumors. The presence of antigen was monitored by measuring the interferon-γ production of BDC-5.2.9 in response to chromatographic fractions in the presence of NOD antigen-presenting cells. Peak antigenic fractions were analyzed by ion-trap mass spectrometry, and candidate proteins were further investigated throu

SUBMITTER: Delong T 

PROVIDER: S-EPMC3161333 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature