Examining smoking-induced differential gene expression changes in buccal mucosa.

ABSTRACT:

Background

Gene expression changes resulting from conditions such as disease, environmental stimuli, and drug use, can be monitored in the blood. However, a less invasive method of sample collection is of interest because of the discomfort and specialized personnel necessary for blood sampling especially if multiple samples are being collected. Buccal mucosa cells are easily collected and may be an alternative sample material for biomarker testing. A limited number of studies, primarily in the smoker/oral cancer literature, address this tissue's efficacy as an RNA source for expression analysis. The current study was undertaken to determine if total RNA isolated from buccal mucosa could be used as an alternative tissue source to assay relative gene expression.

Methods

Total

SUBMITTER: Kupfer DM 

PROVIDER: S-EPMC3161387 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature