High-speed microscopic imaging of flagella motility and swimming in Giardia lamblia trophozoites.

ABSTRACT: We report, in this paper, several findings about the swimming and attachment mechanisms of Giardia lamblia trophozoites. These data were collected using a combination of a high-contrast CytoViva imaging system and a particle image velocimetry camera, which can capture images at speeds greater than 800 frames/s. Using this system, we discovered that, during rapid swimming of Giardia trophozoites, undulations of the caudal region contributed to forward propulsion combined with the beating of the flagella pairs. It was also discovered, in contrast to previous studies with 10 times slower image sampling technique, that the anterior and posterolateral flagella beat with a clearly defined power stroke and not symmetrical undulations. During the transition from free swimming to attachment, tropho

SUBMITTER: Lenaghan SC 

PROVIDER: S-EPMC3161553 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature