Global chromosomal structural instability in a subpopulation of starving Escherichia coli cells.

ABSTRACT: Copy-number variations (CNVs) constitute very common differences between individual humans and possibly all genomes and may therefore be important fuel for evolution, yet how they form remains elusive. In starving Escherichia coli, gene amplification is induced by stress, controlled by the general stress response. Amplification has been detected only encompassing genes that confer a growth advantage when amplified. We studied the structure of stress-induced gene amplification in starving cells in the Lac assay in Escherichia coli by array comparative genomic hybridization (aCGH), with polymerase chain reaction (pcr) and DNA sequencing to establish the structures generated. About 10% of 300 amplified isolates carried other chromosomal structural change in addition to amplification. Most of

SUBMITTER: Lin D 

PROVIDER: S-EPMC3161906 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature