Preferential DNA damage prevention by the E. coli AidB gene: A new mechanism for the protection of specific genes.

ABSTRACT: aidB is one of the four genes of E. coli that is induced by alkylating agents and regulated by Ada protein. Three genes (ada, alkA, and alkB) encode DNA repair proteins that remove or repair alkylated bases. However, the role of AidB remains unclear despite extensive efforts to determine its function in cells exposed to alkylating agents. The E. coli AidB protein was identified as a component of the protein complex that assembles at strong promoters. We demonstrate that AidB protein preferentially binds to UP elements, AT rich transcription enhancer sequences found upstream of many highly expressed genes, several DNA repair genes, and housekeeping genes. AidB allows efficient transcription from promoters containing an UP element upon exposure to a DNA methylating agent and protects downstr

SUBMITTER: Rippa V 

PROVIDER: S-EPMC3162126 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature