SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR.

ABSTRACT:

Background

Real-time PCR can be carried out using either probes or DNA dyes. SYBR Green has been used the most, but it suffers from several drawbacks. Numerous other DNA dyes are commercially available, but with limited structural information. Dye behavior in real time PCR is difficult to predict, so empirical data are needed. In the work described here, a panel of 23 different DNA dyes--including green, orange, and red SYTO dyes, EvaGreen, and SYBR Green--were evaluated with respect to their performance in real time PCR.

Findings

Data were analyzed for reaction inhibition, effects on amplicon melting temperature, fluorescent signal strength, and reaction efficiency. This is the first report of reaction efficiency using alternatives to SYBR Green. Results indicated substanti

SUBMITTER: Eischeid AC 

PROVIDER: S-EPMC3162529 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature