Identification of a phosphorylation-dependent nuclear localization motif in interferon regulatory factor 2 binding protein 2.

ABSTRACT:

Background

Interferon regulatory factor 2 binding protein 2 (IRF2BP2) is a muscle-enriched transcription factor required to activate vascular endothelial growth factor-A (VEGFA) expression in muscle. IRF2BP2 is found in the nucleus of cardiac and skeletal muscle cells. During the process of skeletal muscle differentiation, some IRF2BP2 becomes relocated to the cytoplasm, although the functional significance of this relocation and the mechanisms that control nucleocytoplasmic localization of IRF2BP2 are not yet known.

Methodology/principal findings

Here, by fusing IRF2BP2 to green fluorescent protein and testing a series of deletion and site-directed mutagenesis constructs, we mapped the nuclear localization signal (NLS) to an evolutionarily conserved sequence (354)ARKRKPSP(3

SUBMITTER: Teng AC 

PROVIDER: S-EPMC3162591 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature