Identification of azurocidin as a potential periodontitis biomarker by a proteomic analysis of gingival crevicular fluid.

ABSTRACT:

Background

The inflammatory disease periodontitis results in tooth loss and can even lead to diseases of the whole body if not treated. Gingival crevicular fluid (GCF) reflects the condition of the gingiva and contains proteins transuded from serum or cells at inflamed sites. In this study, we aimed to discover potential protein biomarkers for periodontitis in GCF proteome using LC-MS/MS.

Results

We identified 305 proteins from GCF of healthy individuals and periodontitis patients collected using a sterile gel loading tip by ESI-MS/MS coupled to nano-LC. Among these proteins, about 45 proteins were differentially expressed in the GCF proteome of moderate periodontitis patients when compared to the healthy individuals. We first identified azurocidin in the GCF, but not the sa

SUBMITTER: Choi YJ 

PROVIDER: S-EPMC3162872 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature