Cloning and expression profiling of the VLDLR gene associated with egg performance in duck (Anas platyrhynchos).

ABSTRACT:

Background

The very low density lipoprotein receptor gene (VLDLR), a member of the low density lipoprotein receptor (LDLR) gene family, plays a crucial role in the synthesis of yolk protein precursors in oviparous species. Differential splicing of this gene has been reported in human, rabbit and rat. In chicken, studies showed that the VLDLR protein on the oocyte surface mediates the uptake of yolk protein precursors into growing oocytes. However, information on the VLDLR gene in duck is still scarce.

Methods

Full-length duck VLDLR cDNA was obtained by comparative cloning and rapid amplification of cDNA ends (RACE). Tissue expression patterns were analysed by semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR). Association between the different genotyp

SUBMITTER: Wang C 

PROVIDER: S-EPMC3162882 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature