Protection-free one-pot synthesis of 2'-deoxynucleoside 5'-triphosphates and DNA polymerization.

ABSTRACT: By differentiating the functional groups on nucleosides, we have designed and developed a one-pot synthesis of deoxyribonucleoside 5'-triphosphates without any protection on the nucleosides. A facile synthesis is achieved by generating an in situ phosphitylating reagent that reacts selectively with the 5'-hydroxyl groups of the unprotected nucleosides. The synthesized triphosphates are of high quality and can be effectively incorporated into DNAs by DNA polymerase. This novel approach is straightforward and cost-effective for triphosphate synthesis.

SUBMITTER: Caton-Williams J 

PROVIDER: S-EPMC3163101 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature