Network for activation of human endothelial cells by oxidized phospholipids: a critical role of heme oxygenase 1.

ABSTRACT:

Rationale

Oxidized palmitoyl arachidonyl phosphatidylcholine (Ox-PAPC) accumulates in atherosclerotic lesions, is proatherogenic, and influences the expression of more than 1000 genes in endothelial cells.

Objective

To elucidate the major pathways involved in Ox-PAPC action, we conducted a systems analysis of endothelial cell gene expression after exposure to Ox-PAPC.

Methods and results

We used the variable responses of primary endothelial cells from 149 individuals exposed to Ox-PAPC to construct a network that consisted of 11 groups of genes, or modules. Modules were enriched for a broad range of Gene Ontology pathways, some of which have not been identified previously as major Ox-PAPC targets. Further validating our method of network construction, modules were con

SUBMITTER: Romanoski CE 

PROVIDER: S-EPMC3163234 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature